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CD16b / FCGR3B / FcRIIIb Antibody Datasheet
 
 
 
 
 
 
 
 
 
Order or Inquire for CD16b Antibody product
 
 
 
 
 
 
 
Detection limit is 20 ng/lane in WB
 
 
 
 
 
 
CD16b / FCGR3B / FcRIIIb抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human CD16b protein ( Catalog#11046-H08H )
 
 
 
 
抗体类型: 
 
Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
 
 
 
 
克隆号 :
 
 
 
9A4D11C5
 
 
 
 
抗体宿主 :
 
 
Mouse IgG2a
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD16b ( rh CD16b; Catalog#11046-H08H ; NP_000561.3 ; Met 1- Ser 200 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
CD16b / FCGR3B / FcRIIIb抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human CD16b / FCGR3B
 
 
 
 
 
 
 
No cross-reactivity in WB and ELISA with
 
Human CD16a / FCGR3A
 
Human CD32a / FCGR2A
 
Human CD32b / FCGR2B
 
Human cell lysate (293 cell line)
 
 
 
 
Western blot : 
 
This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD16b in WB. Using a DAB detection system, the detection limit for human CD16b is approximately 20 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
CD16b / FCGR3B / FcRIIIb Antibody Related Products & Topics
 
 
相关研究领域:
 
Cancer>>Cancer Stem Cell (CSC)>>CD16
 
Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Lineage Markers>> CD16
 
Immunology>>Cluster of Differentiation>>Granulocyte CD Antigen>>Granulocyte Markers / Neutrophils>>CD16
 
Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers / Monocyte Markers>>CD16
 
Immunology>>Cluster of Differentiation>>Natural Killer (NK) Cell CD Antigen>>CD16
 
Immunology>>Fc Receptor>>CD16
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
CD16a
 
Human
 
CD16a Protein, Recombinant
 
10389-H08H
 
 
 
CD16b
 
Human
 
CD16b Protein, Recombinant
 
11046-H08H
 
 
 
CD16-2
 
Mouse
 
CD16-2/FCGR4 Protein, Recombinant
 
50036-M08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human 
 CD16a
 
WB 
 
Mouse Monoclonal Antibody 
 
10389-MM01
 
 
 
Human 
 CD16a
 
ELISA
 
Mouse Monoclonal Antibody
 
10389-MM02
 
 
 
Human 
 CD16a
 
ELISA
 
Mouse Monoclonal Antibody
 
10389-MM03
 
 
 
Human 
 CD16b
 
ELISA
 
Mouse Monoclonal Antibody
 
11046-MM01
 
 
 
Human 
 CD16b
 
WB
 
Mouse Monoclonal Antibody
 
11046-MM04
 
 
 
Human 
 CD16b
 
WB
 
Mouse Monoclonal Antibody
 
11046-MM09
 
 
 
Human 
 CD16b
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
11046-RP01
 
 
 
Human 
 CD16b
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
11046-RP02
 
 
 
Mouse 
 CD16-2
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
50036-RP01
 
 
 
Mouse 
 CD16-2
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
50036-RP02
 
 
 
 
 
 CD16b / FCGR3B / FcRIIIb抗体背景综述
 
 
CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide-anchored form (CD16a) in human natural killer cells and macrophages and as a GPI-anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.
 
 
参考文献
 
 
 
Nagarajan, S. et al., 1995, J Biol Chem. 270 (43): 25762-70.
 
Middelhoven, PJ. et al., 1999, Biochem Biophys Res Commun. 255 (3):568-74.
 
Williams, TE. et al., 2000, Biophys J. 79 (4): 1867-75.
 
Fernandes, M. J. et al., 2006, Biochem J. 393 (Pt 1): 351-9.
 
 
 
 
 
 
 
 
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