Human Survivin Quantikine ELISA Kit|R&D|DSV00
产品名称： Human Survivin Quantikine ELISA Kit|R&D|DSV00
英文名称： Human Survivin Quantikine ELISA Kit
产品编号： DSV00
产品价格： 0
产品产地： 美国
品牌商标： R&D
更新时间： null
使用范围： null
- Assay TypeSolid Phase Sandwich ELISA
- Format96-well strip plate
- Assay Length4.5 hours
- Sample Type & Volume Required Per WellCell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
- Sensitivity9.96 pg/mL
- Assay Range31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
- SpecificityNatural and recombinant human Survivin
- Cross-reactivity< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
- InterferenceNo significant interference observed with available related molecules.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 190 | 513 | 982 | 197 | 529 | 1060 |
| Standard Deviation | 7.78 | 17.4 | 53.8 | 18.8 | 37.3 | 59.9 |
| CV% | 4.1 | 3.4 | 5.5 | 9.5 | 7.1 | 5.6 |
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 137 | 398 | 841 | 164 | 445 | 879 |
| Standard Deviation | 6.41 | 27.3 | 44.2 | 14.6 | 39.4 | 63.8 |
| CV% | 4.7 | 6.9 | 5.3 | 8.9 | 8.9 | 7.3 |
The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 88-109 |
| EDTA Plasma (n=4) | 96 | 89-109 |
| Heparin Plasma (n=4) | 96 | 87-110 |
| Serum (n=4) | 93 | 85-109 |
- StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.
- Entrez Gene IDs332 (Human);
- Alternate NamesAPI4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4; Survivin;