PRKCA polyclonal antibody
产品名称： PRKCA polyclonal antibody
英文名称： PRKCA polyclonal antibody
产品编号： PAB4531
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCA.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRKCA.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Protein kinase C-alpha phosphorylation of specific serines in the connecting segment of the beta 4 integrin regulates the dynamics of type II hemidesmosomes.
Rabinovitz I, Tsomo L, Mercurio AM.Mol Cell Biol. 2004 May;24(10):4351-60.
- 2.
- Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells.
Gong N, Wei H, Chowdhury SH, Chatterjee S.Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6490-5. Epub 2004 Apr 14.
- 3.
- Cell volume regulation in response to hypotonicity is impaired in HeLa cells expressing a protein kinase Calpha mutant lacking kinase activity.
Hermoso M, Olivero P, Torres R, Riveros A, Quest AF, Stutzin A.J Biol Chem. 2004 Apr 23;279(17):17681-9. Epub 2004 Feb 11.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of PRKCA polyclonal antibody (Cat # PAB4531) in placenta lysate . PRKCA (arrow) was detected using purified PRKCA polyclonal antibody (Cat # PAB4531) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCA polyclonal antibody (Cat # PAB4531) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Entrez GeneID:
- 5578
- Protein Accession#:
- P17252
- Gene Name:
- PRKCA
- Gene Alias:
- AAG6,MGC129900,MGC129901,PKC-alpha,PKCA,PRKACA
- Gene Description:
- protein kinase C, alpha
- Omim ID:
- 176960
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
- Other Designations:
- aging-associated gene 6,protein kinase C alpha type
- Gene Pathway
- Calcium signaling pathway
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Leukocyte transendothelial migration
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Natural killer cell mediated cytotoxicity
- Non-small cell lung cancer
- Pathogenic Escherichia coli infection - EHEC
- Pathways in cancer
- Phosphatidylinositol signaling system
- Tight junction
- Vascular smooth muscle contraction
- VEGF signaling pathway
- Vibrio cholerae infection
- Wnt signaling pathway