RNF25 polyclonal antibody
产品名称： RNF25 polyclonal antibody
英文名称： RNF25 polyclonal antibody
产品编号： PAB11256
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RNF25.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of human RNF25.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:40000)
Western Blot (1:200-1:2000)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
Asamitsu K, Tetsuka T, Kanazawa S, Okamoto T.J Biol Chem. 2003 Jul 18;278(29):26879-87. Epub 2003 May 13.
- 2.
- RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.
Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM.Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11364-9.
- Applications
- Western Blot (Transfected lysate)
- Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
- Entrez GeneID:
- 64320
- Protein Accession#:
- NP_071898;Q96BH1
- Gene Name:
- RNF25
- Gene Alias:
- AO7,FLJ13906
- Gene Description:
- ring finger protein 25
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
- Other Designations:
- ring finger protein AO7