MFN2 monoclonal antibody (M03), clone 4H8
产品名称： MFN2 monoclonal antibody (M03), clone 4H8
英文名称： MFN2 monoclonal antibody (M03), clone 4H8
产品编号： H00009927-M03
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant MFN2.
- Immunogen:
- MFN2 (NP_055689, 661 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- FKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.41 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study.
Cosson P, Marchetti A, Ravazzola M, Orci L.PLoS One. 2012;7(9):e46293. doi: 10.1371/journal.pone.0046293. Epub 2012 Sep 28.
- 2.
- Mitochondrial bioenergetics and dynamics interplay in complex i-deficient fibroblasts.
Moran M, Rivera H, Sanchez-Arago M, Blazquez A, Merinero B, Ugalde C, Arenas J, Cuezva JM, Martin MA.Biochim Biophys Acta. 2010 May;1802(5):443-453. Epub 2010 Feb 11.
- 3.
- PGC1{alpha} relationship with skeletal muscle palmitate oxidation is not present with obesity, despite maintained ained PGC1{alpha} and PGC1{beta} protein.
Holloway GP, Perry CG, Thrush AB, Heigenhauser GJ, Dyck DJ, Bonen A, Spriet LL.Am J Physiol Endocrinol Metab. 2008 Jun;294(6):E1060-9. Epub 2008 Mar 18.
- Applications
- Western Blot (Cell lysate)
- MFN2 monoclonal antibody (M03), clone 4H8 Western Blot analysis of MFN2 expression in HeLa ( Cat # L013V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- MFN2 monoclonal antibody (M03), clone 4H8. Western Blot analysis of MFN2 expression in PC-12 ( Cat # L012V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8.
Lane 1: MFN2 transfected lysate(86.4 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 5 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged MFN2 is approximately 1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 9927
- GeneBank Accession#:
- NM_014874
- Protein Accession#:
- NP_055689
- Gene Name:
- MFN2
- Gene Alias:
- CMT2A,CMT2A2,CPRP1,HSG,KIAA0214,MARF
- Gene Description:
- mitofusin 2
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq
- Other Designations:
- OTTHUMP00000002509,hyperplasia suppressor,mitochondrial assembly regulatory factor,mitofusin-2,transmembrane GTPase MFN2