• Product name

  EliKine™ Human GM-CSF ELISA Kit

• Reactivity

  Human

• Applications

  ELISA

• Application notes

  EliKine™ Human GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Detection method

  Colorimetric

• Sample type

  Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

  Sandwich ELISA (quantitative)

• Assay duration

  Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human GM-CSF Standard Curve.

Fig.2. EliKine™ Human GM-CSF ELISA Kit is a sandwich ELISA to quantitate GMCSF in samples.

Product Properties

• Kit components

  • Human GM-CSF microplate
  • Human GM-CSF standard
  • Human GM-CSF detect antibody
  • Avidin-HRP
  • Standard diluent
  • Assay buffer
  • HRP substrate
  • Stop solution
  • Wash buffer
  • Plate covers.

• Features & Benefits

  EliKine™ Human GM-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Human GM-CSF. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.

• Calibration range

  11.72 pg/mL-750 pg/mL

• Limit of detection

  5 pg/mL

• Usage notes

  • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
  • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
  • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
  • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
  • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
  • It is recommended that all samples and standards be assayed in duplicate or triplicate.

• Storage instructions

  The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).

• Gene ID

  1437

• Alternative names

  GM-CSF; GMCSF; Colony stimulating factor; Granulocyte Macrophage Colony Stimulating Factor; CSF 2; CSF2

• Accession

  P04141