P53是著名的细胞基因组卫士，也是一种重要的肿瘤抑制基因，自从P53基因于1979年被首次报道后，SCI收录的有关研究论文已达数万篇。目前，在人类各类肿瘤中P53突变率超过50%，是肿瘤中最常见的遗传学改变。这意味着该基因的改变在人类肿瘤的发生、发展以及诊断、治疗等方面具有重要意义。 博奥森精品P53抗体，每个产品开放20支试用申请，先到先得......
本次活动试用抗体 每个产品限20支试用装
| 靶标 | 编号 | 交叉反应 | 应用 |
| P53 | bsm-10841M | Hu | ELISA IHC |
|
P53 protein (wt-p53) |
bs-0033R |
Hu Mo Rat |
ELISA ICC |
|
P53(wt-p53) (D2F7) |
bsm-2346M | Hu Mo |
WB ELISA |
|
phospho-P53 (Ser33) |
bs-3705R | Hu Mo |
WB ELISA IHC ICC FC |
|
phospho-P53 (Thr55) |
bs-5596R | Hu |
ELISA ICC FC |
抗体数据展示 LOVO cell (Human)lysate at 30μg were subjected to SDS-PAGE followed by WB with bs-5596R (Anti-Thr55 phospho p53) at dilution of 1:1000 incubated at 4℃ overnight. Colon carcinoma (Human, 1) and kidney (Mouse, 2) lysate at 40μg were subjected to SDS-PAGE followed by WB with bsm-2346M (Anti-p53-wt) at dilution of 1:5000 incubated at 4℃ overnight.
Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (P53 protein(wt-p53)) polyclonal Antibody, Unconjugated (bs-0033R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.